Document Type : Original Article
Authors
1
M.Sc. Students, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
2
Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
3
Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
4
Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
5
Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran.
Abstract
Abstract
Objective
The date palm (Phoenix dactylifera L.) is a monocotyledonous Arecaceae family plant widely cultivated in arid and semi-arid regions, including Iran. This investigation used the trnH-psbA intergenic region to study the intraspecific genetic diversity of 15 date palm cultivars in the southern regions of Sistan and Baluchestan province.
Materials and Methods
After sampling fresh leaves, DNA was extracted using the Dellaporta method. PCR was then performed using primers specific to the trnH-psbA genes’ region. Next, the amplicons were sent for sequencing.
Results
After receiving the sequencing results, the sequence quality was reviewed using the Chromas software and aligned using BioEdit and MEGA7. According to the analysis of the sequences, a total of 787 polymorphic positions were identified in the trnH-psbA intergenic region. The average value of synonymous and nonsynonymous substitutions (dN/dS) was approximately 0.25, indicating positive and purifying selection in the natural selection process of the studied varieties. The phylogenetic dendrogram and sequence similarity matrix revealed that the cultivars Gozloo 11 and Shand_Shekand 10 from Saravan exhibited the most significant genetic distance, with a coefficient of 0.046. Based on the dendrogram obtained from cluster analysis, the studied cultivars were classified into four distinct groups. Gazalu 11 from Saravan was placed in the first group, Shandeshkand 10 from Saravan in the second group, Peymazo 16 from Jaleq in the third group, and the remaining 12 date genotypes were placed in the fourth group.
Conclusion
This research indicates that the trnH-psbA marker is suitable for investigating the intrageneric diversity of different Date Palm cultivars. However, further investigation is needed to obtain more comprehensive data. It is also recommended that the diversity of this cultivar be studied using other DNA barcodes and appropriate molecular markers.
Keywords
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