نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران.
2 دانشیار، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران.
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Abstract
Objective
MicroRNAs are small, non-coding RNA molecules that play a role in regulating gene expression. MicroRNAs are transcribed by RNA polymerase II to generate precursors that undergo a series of cleavage events to form mature microRNAs. These small non-coding RNA molecules perform their regulatory role through the RISC complex. MicroRNAs play an important role in various plant processes, including growth, development, and response to biotic and abiotic stresses. This study aims to identify conserved microRNAs and their target genes in Capsella rubella, an annual plant known for its anti-contraction and anti-inflammatory effects.
Materials and Methods
MicroRNA homologs in C. rubella were identified using Arabidopsis microRNAs. The stem-loop structures generating mature microRNAs were predicted, the locations of the mature microRNAs on these structures were determined, and potential target genes for each microRNA were predicted.
Results
Three conserved microRNAs were predicted: Cru-miR156, Cru-miR169, and Cru-miR171. A total of 12 potential target genes were identified for these three microRNAs. These genes are mainly transcription factors involved in various important plant processes, such as the transition from the vegetative to the reproductive phase and responses to stresses.
Conclusion
No microRNA has been reported for this plant in the miRBase database, making this the first report of microRNAs and their potential target genes in C. rubella. These findings contribute to a better understanding of the processes regulated by microRNAs in this plant, such as growth, development, and stress responses.
کلیدواژه‌ها [English]
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