نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران.
2 استادیار، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران.
3 دانشیار، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران.
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Abstract
Objective
Cherry tomato varieties are becoming more popular, mainly as fresh produce. Several major genes, such as the lc gene, influence the characteristics of tomato fruit. T. This study aimed to assess the effect of the lc gene on various marketability traits in cherry tomatoes.
Materials and Methods
This study transferred the lc gene into the elite K33 cherry tomato line via backcross breeding. The Pardis cultivar and K33 line were used as donor and recurrent parents, respectively. In the BC1 generation, several marketability traits were measured, including fruit weight, diameter, length, pH, shelf life, pulp weight, pulp volume, and pericarp thickness. Genomic DNA was extracted from leaf samples of all BC1 genotypes. The genotypes were divided into two groups based on PCR results. Subsequently, MANOVA and t-tests were performed to assess the differences between the two genotype groups for the measured traits.
Results
The results of the MANOVA test revealed significant genotypic differences between the two groups for all measured traits. The t-test results showed significant differences between the two groups for fruit weight, diameter, pulp weight, and pulp volume at the 5% significant level. The lc gene-carrying group's mean values were higher than those lacking the lc gene.. However, no significant differences were observed between the groups for fruit length, pH, Shelf life, and pericarp thickness. The mean values of the lc gene-carrying group for fruit weight and diameter were 12.20 g and 2.80 cm, respectively, While the mean values for the group lacking the lc gene were 7.97 g and 2.39 cm, respectively. The mean values for pulp weight and volume in the lc gene-carrying group were 3.64 g and 3.44 cm3, respectively, whereas the corresponding values for the lc gene-lacking group were 1.80 g and 2.08 cm3, respectively.
Conclusion
The lc gene increased fruit pulp weight and size without affecting pH. The consistent pH, combined with increases in fruit weight, diameter, and pulp (53.7%, 17.15%, and 65.38%, respectively), suggests a positive effect on fruit quality.
کلیدواژه‌ها [English]
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