نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانش‌آموخته کارشناسی ارشد، گروه بیوتکنولوژی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران.
2 استادیار، گروه بیوتکنولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران.
3 دانشیار، گروه اکولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران.
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Objective
Diploid wheat (Triticum boeoticum) is a wild relative of cultivated wheat and an important genetic resource. The western provinces of Iran, situated in the eastern part of the Fertile Crescent, are recognized as a key center of diversity for this species. Due to its long-term adaptation to diverse natural environments, T. boeoticum likely harbors valuable genes that contribute to tolerance against various environmental stresses. Given the significance of these genetic resources, the present study aimed to investigate the genetic diversity of selected T. boeoticum populations using Sequence-Related Amplified Polymorphism (SRAP) markers.
Materials and Methods
Ten distinct populations of wild wheat (T. boeoticum) collected from the western provinces of Iran were cultivated under greenhouse conditions. Leaf tissues were harvested for genomic DNA extraction, which was performed using the CTAB method. DNA was amplified using the polymerase chain reaction (PCR) with SRAP primers. The resulting PCR products were separated by agarose gel electrophoresis. After staining, the amplified DNA fragments (bands) were visualized and scored. Genetic diversity among the populations was subsequently analyzed based on the banding patterns.
Results
A total of 114 bands were generated using SRAP markers, all exhibiting an acceptable level of polymorphism. The number of amplified fragments varied across primer combinations. The highest number of bands (14) was observed with primer pairs F3R3 and F3R1, while the lowest (3) was produced by F2R4 and F2R5. Cluster analysis based on the UPGMA method grouped the populations into four distinct clusters. The highest polymorphism information content (PIC) was recorded for primer F1R4, whereas primer F2R5 demonstrated superior ability in distinguishing genetic variation among the studied populations.
Conclusion
Overall, the results indicate that SRAP markers are practical tools for assessing genetic diversity in T. boeoticum.
کلیدواژه‌ها [English]
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